Title

IN VITRO HEMOLYSIS INHIBITION POTENTIAL OF ALUGBATI (Basella alba) ETHANOLIC LEAF EXTRACT ON
DIFFERENT BLOOD TYPES

Author(s)

DANGGA, ALLYZA JANE S.

FULLO, ANTHONY M. II

SULITE, PRINCESS JANE B.

Affiliation

Misamis University

Degree Program

Bachelor of Science in Medical Technology

Contributor

Lao, Karl Maxel O., MSc MLS - Adviser
Alcabaza, Arjade O. , RCht - Co-adviser
Enguito, Marie Roselynn C., MSc Bio - Panel member
Parba, Meliza PhD - Panel member
Villantes, Yunalyn L., PhD - Chairman

Year

2026

Abstract

Basella alba (Malabar spinach) is a commonly consumed medicinal plant known for its antioxidant, anti-inflammatory, and cytoprotective properties. Despite its recognized therapeutic potential, limited studies have investigated its membrane stabilizing effects on human erythrocytes of different blood types. This study evaluated the in vitro membrane-stabilizing activity of ethanolic leaf extract of B. alba on human erythrocytes of blood types A, B, and AB under hypotonic conditions. A quantitative experimental research design was employed, using a hypotonicity-induced hemolysis assay to determine the extract's protective effects on erythrocyte membranes. The extract was tested at concentrations of 1.0, 1.5, and 2.0 mg/mL, with acetylsalicylic acid serving as the positive control and a hypotonic saline–sodium phosphate buffer solution (pH 7.4) without the extract serving as the negative control. The results demonstrated that the ethanolic extract exhibited measurable membrane-stabilizing activity across all tested blood types and concentrations. A concentration-dependent increase in membrane protection was observed, with the 2.0 mg/mL extract showing the greatest inhibition of hemolysis. Among the blood types tested, blood type B exhibited the highest membrane-stabilizing response. Statistical analysis using two-way ANOVA revealed significant differences in membrane-stabilizing activity among extract concentrations and blood type groups (p < .001). These findings suggest that B. alba contains bioactive phytochemicals with potential membrane-protective and cytoprotective properties, supporting its possible therapeutic application as a natural source of anti-inflammatory agents

Keywords

Erythrocyte stability, cytoprotection, hypotonic stress, phytochemicals, therapeutic potential