Title

DETECTION OF FOODBORNE BACTERIA IN COMMERCIALLY
SOLD ANINIKAD (Canarium urceus) IN TUDELA, MISAMIS OCCIDENTAL

Author(s)

MARASIGAN, KASSANDRA CORINNE Y.

PINATACAN, JOHN PAUL M.

PUTOL, XYLLIENGEN RUTHER P.

SEDEÑO, AUBREY ANN G.

Affiliation

Misamis University

Degree Program

Bachelor of Science in Medical Technology

Contributor

Balasabas, Ruthie M., RMT, MBA-HHA - Adviser
Enguito, Marie Roselynn, C, MSc. Bio - Panel member
Atty. Awa. Anthony L., MSc. Bio,LPT - Panel member
Lao, Karl Maxel O. MSc.MLS - Chairman

Year

2026

Abstract

Foodborne pathogens in seafood remain a significant public health concern, particularly in marine gastropods that may accumulate microorganisms from contaminated coastal environments. This study assessed the microbiological quality of commercially sold aninikad (Canarium urceus) obtained from Tudela, Misamis Occidental by determining the presence and contamination levels of Vibrio spp. and Salmonella spp. A descriptive, cross-sectional research design was employed. Soft tissues from the collected samples were processed and analyzed using standard microbiological procedures in accordance with the U.S. Food and Drug Administration Bacteriological Analytical Manual. Vibrio spp. were isolated using Alkaline Peptone Water and Thiosulfate-Citrate-Bile Salts-Sucrose agar. In contrast, Salmonella spp. were analyzed using Buffered Peptone Water, Rappaport-Vassiliadis broth, and Salmonella-Shigella agar. Colony counts were expressed as colony-forming units per gram (CFU/g) and converted to log10 CFU/g values to evaluate contamination risk. Results showed the presence of Vibrio spp., with V.cholerae identified as the predominant species, while V.parahaemolyticus and Salmonella spp.
were not detected. The bacterial load of V.cholerae had a mean concentration of 1.02 × 107 CFU/g, corresponding to 7.009 ± 0.005 log10 CFU/g. This level exceeded the established threshold for Vibrio spp. and indicated a medium-to-high risk of contamination. The findings demonstrate that commercially sold C. urceus from Tudela may harbor potentially pathogenic Vibrio species and highlight the importance of proper handling, cooking, and routine microbial monitoring to ensure seafood safety and protect public health.

Keywords

Canarium urceus, foodborne pathogens, food safety, microbial contamination, public health