-
Title
-
MICROBIAL IDENTIFICATION AND ANTIMICROBIAL
RESISTANCE OF MICROORGANISMS PRESENT IN LAMP
SHELL (Lingula anatina Lamarck, 1801) AND PEANUT WORM
(Sipunculus nudus Linnaeus, 1766) SEAFOOD SAMPLES
AT BARRA, TUDELA, MISAMIS OCCIDENTAL
-
Author(s)
-
ABAO, RHINNA BETH V.
GARCIA, ZAIRA MICH O.
LABASTILLA,KIRSTEN LEVI V.
LACUATA, JULIANA VANNICE B.
LANAS, GRACE C.
LLOBRERA, EIDNEL JHANE L.
MOTUS, NICHAELA HONEY S.
-
Affiliation
-
MISAMIS UNIVERSITY
-
Degree Program
-
Bachelor of Science in Medical Technology
-
Contributor
-
LAO, KARL MAXEL O. RMT, MSc MLS
VILLANTES, YUNALYN L. MSc BIO, PhD
ENGUITO, MARIE ROSELLYNN MSc BIO
TAYLARAN, DARWIN R. LPT
BALASABAS, RUTHIE M. RMT, MBA-HHA
-
Year
-
2026
-
Abstract
-
Coastal marine ecosystems are increasingly acknowledged as possible sources of antimicrobial-resistant bacteria that could be spread through seafood ingestion. This research analyzed the microbial makeup and antimicrobial resistance (AMR)
characteristics of edible seafood obtained from the coastal waters of Barangay Barra, Tudela, Misamis Occidental, utilizing the lamp shell (Lingula anatina Lamarck, 1801) and peanut worm (Sipunculus nudus Linnaeus, 1766) as biological indicators because of their
strong connection to bottom sediments, where pollutants and resistant microorganisms were known to accumulate. Samples were collected under sterile conditions from Barangay Barra, Tudela, and were processed in the Medical Technology Laboratory of Misamis University. Bacterial isolation, identification, and antibiotic susceptibility testing were carried out utilizing the VITEK system in accordance with Clinical and Laboratory Standards Institute (CLSI) guidelines. A total of 3 bacterial isolates were obtained such as Vibrio alginolyticus, Vibrio parahaemolyticus, and Klebsiella pneumoniae ssp pneumoniae. Antimicrobial testing showed Ampicillin demonstrated the strongest and most consistent resistance across all bacterial isolates from both peanut worm and lamp shell. In contrast, the highest susceptibility profiles were consistently noted in aminoglycosides (Amikacin and Gentamicin), fluoroquinolones (Ciprofloxacin), and advanced cephalosporins (Cefotaxime, Ceftazidime, and Cefepime), which demonstrated
an outstanding susceptibility record across all samples tested. Additionally, Amoxicillin/Clavulanic acid and Piperacillin/Tazobactam remained highly effective,
whereas Meropenem and Trimethoprim/Sulfamethoxazole showed reduced efficacy due to emerging resistance in specific samples. These results indicate possible public health hazards linked to eating contaminated seafood and highlight the necessity for ongoing surveillance of microbial contamination and antimicrobial resistance in coastal waters to ensure seafood safety, safeguard public health, and promote sustainable management of coastal resources.
-
Keywords
-
antibiotics, seafood contamination, Vibrio species, Klebsiella pneumoniae,
VITEK system
